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DNA adsorption studies of poly(4,4'-cychlohexylidene bisphenol oxalate)/silica nanocomposites


Citation

Al Balawi, Aisha Nawaf and Yusof, Nor Azah and Kamaruzaman, Sazlinda and Mohammad, Faruq and Wasoh @ Mohamad Isa, Helmi and Al-Lohedan, Hamad A. (2019) DNA adsorption studies of poly(4,4'-cychlohexylidene bisphenol oxalate)/silica nanocomposites. Materials, 12 (7). art. no. 1178. pp. 1-15. ISSN 1996-1944

Abstract

The present study deals with the synthesis, characterization, and DNA extraction of poly(4,4′-cyclohexylidene bisphenol oxalate)/silica (Si) nanocomposites (NCs). The effects of varying the monomer/Si (3.7%, 7%, and 13%) ratio towards the size and morphology of the resulting NC and its DNA extraction capabilities have also been studied. For the NC synthesis, two different methods were followed, including the direct mixing of poly(4,4′-cyclohexylidene bisphenol oxalate) with fumed Si, and in situ polymerization of the 4,4′-cyclohexylidene bisphenol monomer in the presence of fumed silica (11 nm). The formed NCs were thoroughly investigated by using different techniques such as scanning electron microscopy (SEM), fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), powdered X-ray diffraction (XRD), and Brunauer–Emmett–Teller (BET) analysis where the results supported that there was the successful formation of poly(4,4′-cyclohexylidene bisphenol oxalate)/Si NC. Within the three different NC samples, the one with 13% Si was found to maintain a very high surface area of 12.237 m2/g, as compared to the other two samples consisting of 7% Si (3.362 m2/g) and 3.7% Si (1.788 m2/g). Further, the solid phase DNA extraction studies indicated that the efficiency is strongly influenced by the amount of polymer (0.2 g > 0.1 g > 0.02 g) and the type of binding buffer. Among the three binding buffers tested, the guanidine hydrochloride/EtOH buffer produced the most satisfactory results in terms of yield (1,348,000 ng) and extraction efficiency (3370 ng/mL) as compared to the other two buffers of NaCl (2 M) and phosphate buffered silane. Based on our results, it can be indicated that the developed poly(4,4′-cyclohexylidene bisphenol oxalate)/Si NC can serve as one of the suitable candidates for the extraction of DNA in high amounts as compared to other traditional solid phase approaches.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Institute of Advanced Technology
DOI Number: https://doi.org/10.3390/ma12071178
Publisher: MDPI
Keywords: Poly(4,4′-cyclohexylidene bisphenol oxalate); Surface modification; Silicon; Nanocomposite; DNA extraction; Polymerization
Depositing User: Nabilah Mustapa
Date Deposited: 04 May 2020 16:28
Last Modified: 04 May 2020 16:28
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/ma12071178
URI: http://psasir.upm.edu.my/id/eprint/38402
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