Citation
Abstract
Gluconasturtiin, a glucosinolate present in watercress, is hydrolysed by myrosinase to form gluconasturtiin-isothiocyanate (GNST-ITC), which has potential chemopreventive effects; however, the underlying mechanisms of action have not been explored, mainly in human cell lines. The purpose of the study is to evaluate the cytotoxicity of GNST-ITC and to further assess its potential to induce apoptosis. GNST-ITC inhibited cell proliferation in both human hepatocarcinoma (HepG2) and human breast adenocarcinoma (MCF-7) cells with IC50 values of 7.83 µM and 5.02 µM, respectively. Morphological changes as a result of GNST-ITC-induced apoptosis showed chromatin condensation, nuclear fragmentation, and membrane blebbing. Additionally, Annexin V assay showed proportion of cells in early and late apoptosis upon exposure to GNST-ITC in a time-dependent manner. To delineate the mechanism of apoptosis, cell cycle arrest and expression of caspases were studied. GNST-ITC induced a time-dependent G2/M phase arrest, with reduction of 82% and 93% in HepG2 and MCF-7 cell lines, respectively. The same treatment also led to the subsequent expression of caspase-3/7 and -9 in both cells demonstrating mitochondrial-associated cell death. Collectively, these results reveal that GNST-ITC can inhibit cell proliferation and can induce cell death in HepG2 and MCF-7 cancer cells via apoptosis, highlighting its potential development as an anticancer agent.
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Official URL or Download Paper: https://www.mdpi.com/1420-3049/25/5/1240
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Food Science and Technology Institute of Bioscience Institute of Tropical Agriculture and Food Security |
DOI Number: | https://doi.org/10.3390/molecules25051240 |
Publisher: | MDPI |
Keywords: | Glucosinolate; Gluconasturtiin-isothiocyanate; Apoptosis; Cancer chemoprevention |
Depositing User: | Nabilah Mustapa |
Date Deposited: | 03 May 2020 22:53 |
Last Modified: | 03 May 2020 22:53 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules25051240 |
URI: | http://psasir.upm.edu.my/id/eprint/38128 |
Statistic Details: | View Download Statistic |
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