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Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode


Citation

A. Talib, Nurul Ain and Salam, Faridah and Sulaiman, Yusran (2018) Development of highly sensitive immunosensor for clenbuterol detection by using poly(3,4-ethylenedioxythiophene)/graphene oxide modified screen-printed carbon electrode. Sensors, 18 (12). art. no. 4324. pp. 1-13. ISSN 424-8220

Abstract

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).


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Official URL or Download Paper: https://www.mdpi.com/1424-8220/18/12/4324

Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Institute of Advanced Technology
DOI Number: https://doi.org/10.3390/s18124324
Publisher: MDPI
Keywords: Clenbuterol; Poly(3,4-ethylenedioxythiophene); Graphene oxide; Immunosensor; Electrochemical
Depositing User: Nabilah Mustapa
Date Deposited: 14 Apr 2020 14:01
Last Modified: 14 Apr 2020 14:01
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/s18124324
URI: http://psasir.upm.edu.my/id/eprint/38098
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