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Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources


Citation

Mohamed Shakrina, Nik Noorul Shakira and Masri, Siti Norbaya and Mohd Taib, Niazlin and Amin Nordin, Syafinaz and Jamal, Farida and Mohd Desa, Mohd Nasir (2014) Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources. Comparative Immunology, Microbiology and Infectious Diseases, 37 (5-6). pp. 347-354. ISSN 0147-9571; ESSN: 1878-1667

Abstract / Synopsis

This study characterized carriage and clinical pneumococcal isolates for serotypes, penicillin susceptibility, virulence genes and restriction fragment length polymorphism (RFLP) pattern of penicillin binding protein (PBP) genes. DNA fingerprint of isolates was generated by BOX-PCR. Majority of serotypes were 23F followed by 19F, 19A and 6A. Twenty-four percent of isolates were penicillin non-susceptible (PNSP). All of the targeted virulence genes were detected in all isolates with the exception of pili; 20.6% (n = 22) for PI-1 and 14.0% (n = 15) for PI-2. Of the 13 isolates which carried both PI-1 and PI-2, 10 were of clinical origin. Digested pbp-DNA produced three PBP-RFLP profiles for pbp1a (A1 to A3), six profiles for pbp2b (B1 to B6) and seven for pbp2x (X1 to X7) mostly in PNSPs. Based on BOX-PCR analysis, the majority of isolates were genetically diverse with a small number of potentially related isolates carrying pili genes. No obvious genotypic association was observed pertaining to carriage and clinical origin of isolates.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Halal Products Research Institute
DOI Number: https://doi.org/10.1016/j.cimid.2014.10.005
Publisher: Elsevier
Keywords: Streptococcus pneumoniae; Serotype; Virulence; Pili; Vaccine; PBP-RFLP; BOX-PCR
Depositing User: Nabilah Mustapa
Date Deposited: 22 Apr 2016 10:38
Last Modified: 22 Apr 2016 10:38
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.cimid.2014.10.005
URI: http://psasir.upm.edu.my/id/eprint/37766
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