Citation
Abstract
This study characterized carriage and clinical pneumococcal isolates for serotypes, penicillin susceptibility, virulence genes and restriction fragment length polymorphism (RFLP) pattern of penicillin binding protein (PBP) genes. DNA fingerprint of isolates was generated by BOX-PCR. Majority of serotypes were 23F followed by 19F, 19A and 6A. Twenty-four percent of isolates were penicillin non-susceptible (PNSP). All of the targeted virulence genes were detected in all isolates with the exception of pili; 20.6% (n = 22) for PI-1 and 14.0% (n = 15) for PI-2. Of the 13 isolates which carried both PI-1 and PI-2, 10 were of clinical origin. Digested pbp-DNA produced three PBP-RFLP profiles for pbp1a (A1 to A3), six profiles for pbp2b (B1 to B6) and seven for pbp2x (X1 to X7) mostly in PNSPs. Based on BOX-PCR analysis, the majority of isolates were genetically diverse with a small number of potentially related isolates carrying pili genes. No obvious genotypic association was observed pertaining to carriage and clinical origin of isolates.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Medicine and Health Science Halal Products Research Institute |
| DOI Number: | https://doi.org/10.1016/j.cimid.2014.10.005 |
| Publisher: | Elsevier |
| Keywords: | Streptococcus pneumoniae; Serotype; Virulence; Pili; Vaccine; PBP-RFLP; BOX-PCR |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 22 Apr 2016 02:38 |
| Last Modified: | 22 Apr 2016 02:38 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.cimid.2014.10.005 |
| URI: | http://psasir.upm.edu.my/id/eprint/37766 |
| Statistic Details: | View Download Statistic |
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