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Functional properties of whey protein concentrate texturized at acidic pH: effect of extrusion temperature


Citation

Mustapha, Nor Afizah and Rizvi, Syed S. H. (2014) Functional properties of whey protein concentrate texturized at acidic pH: effect of extrusion temperature. LWT - Food Science and Technology, 57 (1). pp. 290-298. ISSN 0023-6438

Abstract

Reactive supercritical fluid extrusion (RSCFX) process at acidic condition (pH 3.0) was used to generate texturized whey protein concentrate (TWPC) and the impacts of process temperature on product's physicochemical properties were evaluated. TWPC extruded at 50 and 70 °C formed soft-textured aggregates with high solubility than that extruded at 90 °C that formed protein aggregates with low solubility. Total free sulfhydryl contents and solubility studies in selected buffers indicated that TWPC is primarily stabilized by non-covalent interactions. Proteins texturized at 90 °C showed an increased affinity for 1-anilino-naphthalene-8-sulfonate (ANS) and a decreased affinity for cis-parinaric acid (CPA), indicating changes in protein structure. Water dispersion of TWPC at room temperature showed thickening function with pseudoplastic behavior. Secondary gelation occurred in TWPC obtained at 50 and 70 °C by heating the cold-set gels to 95 °C. TWPC texturized at 90 °C produced cold-set gels with good thermal stability. Compared to control, TWPC formed stable oil-in-water emulsions. Factors such as degree of protein denaturation and the balance of surface hydrophobicity and solubility influenced the heat- and cold-gelation and emulsifying properties of the protein ingredients. TWPC generated by low and high temperature extrusions can thus be utilized for different products requiring targeted physicochemical functionalities.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1016/j.lwt.2014.01.019
Publisher: Elsevier
Keywords: Whey protein; Supercritical fluid extrusion; Protein hydrophobicity; Cold-set gelation; Emulsifying properties
Depositing User: Nabilah Mustapa
Date Deposited: 18 Sep 2015 12:33
Last Modified: 06 Oct 2015 03:36
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.lwt.2014.01.019
URI: http://psasir.upm.edu.my/id/eprint/37724
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