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Ergogenic, anti-diabetic and antioxidant attributes of selected Malaysian herbs: characterisation of flavonoids and correlation of functional activities


Citation

Sallehuddin, N. A. and Abdul Hamid, A. and Salleh, S. Z. and Abdul Majid, N. and Halim, H. H. and Ramli, N. S. and Shukri, R. and Jaafar, A. H. and Hussin, M. and Anwar, F. and Pak Dek, Mohd Sabri (2020) Ergogenic, anti-diabetic and antioxidant attributes of selected Malaysian herbs: characterisation of flavonoids and correlation of functional activities. International Food Research Journal, 27 (1). pp. 197-207. ISSN 1985-4668; ESSN: 2231-7546

Abstract

In the present work, aqueous ethanolic (60% ethanol) extracts from selected Malaysian herbs including Murraya koenigii L. Spreng, Lawsonia inermis L., Cosmos caudatus Kunth, Piper betle L., and P. sarmentosum Roxb. were evaluated for their ergogenic, anti-diabetic and antioxidant potentials. Results showed that the analysed herbs had ergogenic property and were able to activate 5'AMP-activated protein kinase (AMPK) in a concentration dependant manner. The highest AMPK activation was exhibited by M. koenigii extract which showed no significant (p > 0.05) difference with green tea (positive control). For anti-diabetic potential, the highest α-glucosidase inhibition was exhibited by M. koenigii extract with IC50 of 43.35 ± 7.5 μg/mL, which was higher than acarbose (positive control). The determinations of free radical scavenging activity and total phenolics content (TPC) indicated that the analysed herbs had good antioxidant activity. However, C. caudatus extract showed superior antioxidant activity with IC50 against free radical and TPC of 21.12 ± 3.20 μg/mL and 221.61 ± 7.49 mg GAE/g, respectively. RP-HPLC analysis established the presence of flavonoids in the herbs wherein L. inermis contained the highest flavonoid (catechin, epicatechin, naringin and rutin) content (668.87 mg/kg of extract). Correlations between the analyses were conducted, and revealed incoherent trends. Overall, M. koenigii was noted to be the most potent herb for enhancement of AMPK activity and α-glucosidase inhibition but exhibited moderate antioxidant activity. These results revealed that the selected herbs could be potential sources of natural ergogenic and anti-diabetic/antioxidant agents due to their rich profile of phenolics. Further analysis in vivo should be carried out to further elucidate the mechanism of actions of these herbs as ergogenic aids and anti-diabetic/antioxidant agents.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Aqueous ethanol extract; α-glucosidase inhibition; Total phenolics; AMPK activity; RP-HPLC
Depositing User: Nabilah Mustapa
Date Deposited: 06 Jul 2020 03:48
Last Modified: 06 Jul 2020 03:48
URI: http://psasir.upm.edu.my/id/eprint/37571
Statistic Details: View Download Statistic

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