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Evaluation of methane oxidizer bacteria in the rice soil in Malaysia


Citation

Fazli, Pardis and Che Man, Hasfalina and Mohamed Zawawi, Mohamed Azwan and Md. Shah, Umi Kalsom and Abdul Rahman, Nor'aini and Idris, Azni (2014) Evaluation of methane oxidizer bacteria in the rice soil in Malaysia. International Journal of Enhanced Research in Science Technology and Engineering, 3 (3). pp. 360-367. ISSN 2319-7463

Abstract

Methane is known as a powerful greenhouse gas due to its global warming potential (GWP = 21). Rice fields are methane producers because of the flooding irrigation system. Two microbial communities are involved in methane cycle in the soil including methanogens and methanotrophic bacteria which are responsible for methane production and methane oxidation respectively. Methanotrophic bacteria as aerobic unicellular microorganisms dominantly exist in soil oxic area (e.g. surface of the soil and the rhizosphere). These microorganisms can regulate the methane emission from rice soil. This experiment applied PCR-DGGE to detect methane oxidizer bacteria (MOBs) within the rice soil from two depths 0-5 cm and 5-10 cm in different rice growth stages and cultivation systems. Consequently, several MOBs from type I and type II could be identified. However, type I was detected in depth of 0-5 cm and drained condition rather than 5-10 cm and flooding condition.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Engineering
Publisher: ER Publications
Keywords: Methane oxidizer bacteria (MOB); Rice; Denaturing gradient gel electrophoresis (DGGE); Tropical soil
Depositing User: Nabilah Mustapa
Date Deposited: 14 Sep 2015 10:50
Last Modified: 02 Sep 2016 06:33
URI: http://psasir.upm.edu.my/id/eprint/37535
Statistic Details: View Download Statistic

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