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Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21


Citation

Maidin, Mohd Shawal Thakib and Song, Adelene Ai Lian and Jalilsood, Tannaz and Sieo, Chin Chin and Yusoff, Khatijah and Abdul Rahim, Raha (2014) Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21. Plasmid, 74. pp. 32-38. ISSN 0147-619X; ESSN:1095-9890

Abstract

A vector that drives the expression of the reporter gusA gene in both Lactobacillus plantarum and Lactococcus lactis was constructed in this study. This vector contained a newly characterized heat shock promoter (Phsp), amplified from an Enterococcus faecium plasmid, pAR6. Functionality and characterization of this promoter was initially performed by cloning Phsp into pNZ8008, a commercial lactococcal plasmid used for screening of putative promoters which utilizes gusA as a reporter. It was observed that Phsp was induced under heat, salinity and alkaline stresses or a combination of all three stresses. The newly characterized Phsp promoter was then used to construct a novel Lactobacillus vector, pAR1801 and its ability to express the gusA under stress-induced conditions was reproducible in both Lb. plantarum Pa21 and L. lactis M4 hosts.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.plasmid.2014.05.003
Publisher: Elsevier
Keywords: Heat shock promoter; LAB; Lactococcus lactis; Lactobacillus plantarum
Depositing User: Nabilah Mustapa
Date Deposited: 12 Jan 2016 00:26
Last Modified: 12 Jan 2016 00:26
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.plasmid.2014.05.003
URI: http://psasir.upm.edu.my/id/eprint/36892
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