Citation
Abstract
Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.
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Official URL or Download Paper: http://www.hindawi.com/journals/bmri/2014/653014/
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Veterinary Medicine Institute of Bioscience |
| DOI Number: | https://doi.org/10.1155/2014/653014 |
| Publisher: | Hindawi Publishing Corporation |
| Keywords: | PCR-ELISA; Polymerase chain reaction; Method |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 18 Aug 2015 12:25 |
| Last Modified: | 26 Jan 2016 07:50 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2014/653014 |
| URI: | http://psasir.upm.edu.my/id/eprint/36517 |
| Statistic Details: | View Download Statistic |
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