UPM Institutional Repository

Variables affecting the recombinant phytase production from Escherichia coli DH5a


Mohd Ariff, Rafidah and Abd. Manap, Mohd. Yazid and Ideris, Aini and Kassim, Azhar and Farouk, Abd-El Aziem and Meor Hussin, Anis Shobirin (2015) Variables affecting the recombinant phytase production from Escherichia coli DH5a. Journal of Pure and Applied Microbiology, 9 (spec. May). pp. 257-263. ISSN 0973-7510


Screening for the important variables is part of the optimization study of enzyme production. It is important indetermining the influential variables that give a significant effect on the enzyme production. Thus, the objective of this study was to determine variables that are effecting the recombinant phytase production from Escherichia coli DH5±. Through the application of the Fractional Factorial Design, the variables of seed age, inoculum level, time of induction, L-arabinose concentration and post-induction time were highly significant for the recombinant phytase production as their p-values were less than 0.05.Thus, minimal changes of this factor mat effect recombinant phytase production from E. coli DH5±.

Download File

Full text not available from this repository.

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Food Science and Technology
Publisher: Oriental Scientific Publishing
Keywords: Screening; Variables, Optimization; Recombinant phytase; Fractional factorial
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 12 Feb 2016 02:42
Last Modified: 12 Feb 2016 02:42
URI: http://psasir.upm.edu.my/id/eprint/35969
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item