Citation
Mohd Ariff, Rafidah and Abd. Manap, Mohd. Yazid and Ideris, Aini and Kassim, Azhar and Farouk, Abd-El Aziem and Meor Hussin, Anis Shobirin
(2015)
Variables affecting the recombinant phytase production from Escherichia coli DH5a.
Journal of Pure and Applied Microbiology, 9 (spec. May).
pp. 257-263.
ISSN 0973-7510
Abstract
Screening for the important variables is part of the optimization study of enzyme production. It is important indetermining the influential variables that give a significant effect on the enzyme production. Thus, the objective of this study was to determine variables that are effecting the recombinant phytase production from Escherichia coli DH5±. Through the application of the Fractional Factorial Design, the variables of seed age, inoculum level, time of induction, L-arabinose concentration and post-induction time were highly significant for the recombinant phytase production as their p-values were less than 0.05.Thus, minimal changes of this factor mat effect recombinant phytase production from E. coli DH5±.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture Faculty of Food Science and Technology |
| Publisher: | Oriental Scientific Publishing |
| Keywords: | Screening; Variables, Optimization; Recombinant phytase; Fractional factorial |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 12 Feb 2016 02:42 |
| Last Modified: | 12 Feb 2016 02:42 |
| URI: | http://psasir.upm.edu.my/id/eprint/35969 |
| Statistic Details: | View Download Statistic |
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