Citation
Heidary, Somayyeh and Abdul Rahim, Raha and Eissazadeh, Samira and Moeini, Hassan and Leow, Adam Thean Chor and Abdullah, Mohd Puad
(2014)
Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b.
Biotechnology Letters, 36 (7).
pp. 1479-1484.
ISSN 0141-5492; ESSN: 1573-6776
Abstract
The periplasmic proteome of recombinant E. coli cells expressing human interferon-α2b (INF-α2b) was analysed by 2D-gel electrophoresis to find the most altered proteins. Of some unique up- and down-regulated proteins in the proteome, ten were identified by MS. The majority of the proteins belonged to the ABC transporter protein family. Other affected proteins were ones involved in the regulation of transcription such as DNA-binding response regulator, stress-related proteins and ecotin. Thus, the production of INF-α2b acts as a stress on the cells and results in the induction of various transporters and stress related proteins.
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Official URL or Download Paper: http://link.springer.com/article/10.1007/s10529-01...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Institute of Bioscience |
| DOI Number: | https://doi.org/10.1007/s10529-014-1504-7 |
| Publisher: | Springer |
| Keywords: | ABC transporter proteins; DNA-binding response regulator; Escherichia coli; Human interferon α2b; Periplasmic proteome; Stress-related proteins |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 11 Oct 2016 02:22 |
| Last Modified: | 11 Oct 2016 02:22 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10529-014-1504-7 |
| URI: | http://psasir.upm.edu.my/id/eprint/35096 |
| Statistic Details: | View Download Statistic |
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