Citation
Abdu, Hamisu and Ahmad @ Amat, Faujan and Basri, Mahiran and Ismail, Intan Safinar and Abdul Rahman, Mohd Basyaruddin
(2014)
Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435.
Asian Journal of Research in Chemistry, 7 (7).
pp. 640-643.
ISSN 0974-4169; ESSN: 0974-4150
Abstract
The enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-β-D-glucopyranoside betulinic acid was obtained using 30.67 h, 54.30°C and 180 mg of enzymes using betulinic acid (0.05 mmol) and glucose (0. 1 mmol) respectively. The actual percentage yield under these conditions was 88.69 % and the predicted value was 89.05 %.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Science |
| Publisher: | A & V Publications |
| Keywords: | Enzymes; Enzymatic synthesis; 3-O-β-D-glucopyranoside betulinic acid; Novozyme-435; Optimization |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 22 Dec 2015 11:53 |
| Last Modified: | 09 Oct 2018 03:07 |
| URI: | http://psasir.upm.edu.my/id/eprint/34770 |
| Statistic Details: | View Download Statistic |
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