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Strain differences in deconjugation of bile acids in Bifidobacterium pseudocatenulatum isolates


Citation

Sabet, Maryam Rezaei and Yap, Keng Wai and Lim, Long Chang and Syed Muhammad, Sharifah Kharidah and Mustafa, Shuhaimi and Sipat, Abdullah and Ali, Abdul Manaf and Abdullah, Nur Atiqah and Abd Manap, Mohd Yazid (2004) Strain differences in deconjugation of bile acids in Bifidobacterium pseudocatenulatum isolates. Bioscience and Microflora, 23 (2). pp. 93-98. ISSN 1342-1441; ESSN: 1349-8355

Abstract

The survival and growth rate of twenty eight isolates of bifidobacteria in bile were evaluated. Of 28 isolates, 25 were tolerance towards 2.0% concentration of bile while 14 isolates were tolerance towards 4.0% of bile after 12 hours of exposure. Six isolates of bifidobacteria with higher tolerance to 4.0% of bile were further evaluated for their ability to deconjugate different types of bile acids namely taurocholic acid (TC), glycocholic acid (GC), taurochenodeoxycholic acid (TCDC), glycochenodeoxycholic acid (GCDC), taurodeoxycholic acid (TDC), and glycodeoxycholic acid (GDC). Three Bifidobacterium pseudocatenulatum isolates (D22, F117, and G4) were found to have the similar deconjugation activity and were able to deconjugate 78.6-84.6% (TC), 98.9-99.9% (GC), 87.9-97.5% (TCDC), 91.1-100.0% (GCDC), 83.7-87.8% (TDC) and 96.5-99.0% (GDC).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Institute of Bioscience
Publisher: The Japan Bifidus Foundation
Keywords: Bifidobacterium spp.; Probiotic; Deconjugation; Bile salt hydrolase
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 19 Sep 2016 09:27
Last Modified: 19 Sep 2016 09:27
URI: http://psasir.upm.edu.my/id/eprint/34706
Statistic Details: View Download Statistic

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