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Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells


Citation

Ismail, Norsharina and Akhtar, Muhammad Nadeem and Ismail, Maznah and Zareen, Seema and Shah, Syed Adnan Ali and Lajis, Nordin and Tajuddin, Saiful Nizam (2015) Neuroprotective effect from stem bark extracts of Knema laurina against H₂O₂- and Aβ(1-42)-induced cell death in human SH-SY5Y cells. Natural Product Research, 29 (16). pp. 1571-1574. ISSN 1478-6419; ESSN: 1478-6427

Abstract

The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid β-peptide 1–42 length (Aβ1–42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 μM H2O2 or 20 μM Aβ1–42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 μg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 μg/mL exerted neuroprotective effect on Aβ1–42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Aβ1–42-induced cell death. Flavonoids (3–6) and β-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects.


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Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
DOI Number: https://doi.org/10.1080/14786419.2014.985676
Publisher: Taylor & Francis
Keywords: Neuroprotective; Knema laurina; Hydrogen peroxide; Aβ1–42; SH-SY5Y cell; Flavonoids
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 16 Dec 2015 07:46
Last Modified: 16 Dec 2015 07:46
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/14786419.2014.985676
URI: http://psasir.upm.edu.my/id/eprint/34639
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