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Optimal conditions for hepatitis B core antigen production in shaked flask fermentation


Citation

Tey, Beng Ti and Yong, Kok Hoe and Ong, Hong Puay and Ling, Tau Chuan and Ong, Swee Tin and Tan, Yan Peng and Ariff, Arbakariya and Tan, Wen Siang (2004) Optimal conditions for hepatitis B core antigen production in shaked flask fermentation. Biotechnology and Bioprocess Engineering, 9 (5). pp. 374-378. ISSN 1226-8372; ESSN: 1976-3816

Abstract

The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and 40°C) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present study. The highest growth rate is achieved at PH 7, 37°C and at a rotational speed of 250 rpm which is 0.927 h−1. The effect of pH on cell growth is more substantial compared to other parameters; it recorded a 123% different between the highest growth rate (0.927 h−1) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and 40°C. The yield of protein at pH 7 is 154% higher compared to the lowest yield achieved at pH 5. There is about 28% different of the protein yield for theE. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at 40°C is 38% higher compared to the lowest yield achieved, at 30°C.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Faculty of Food Science and Biotechnology
Faculty of Science and Environmental Studies
DOI Number: https://doi.org/10.1007/BF02933060
Publisher: Korean Society for Biotechnology and Bioengineering
Keywords: E. coli; Fermentation; HBcAg; pH; Rotational speed; Temperature
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 19 Sep 2016 09:45
Last Modified: 19 Sep 2016 09:45
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/BF02933060
URI: http://psasir.upm.edu.my/id/eprint/34502
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