Citation
Abstract
The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and 40°C) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present study. The highest growth rate is achieved at PH 7, 37°C and at a rotational speed of 250 rpm which is 0.927 h−1. The effect of pH on cell growth is more substantial compared to other parameters; it recorded a 123% different between the highest growth rate (0.927 h−1) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and 40°C. The yield of protein at pH 7 is 154% higher compared to the lowest yield achieved at pH 5. There is about 28% different of the protein yield for theE. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at 40°C is 38% higher compared to the lowest yield achieved, at 30°C.
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Official URL or Download Paper: http://link.springer.com/article/10.1007/BF0293306...
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Engineering Faculty of Food Science and Biotechnology Faculty of Science and Environmental Studies |
DOI Number: | https://doi.org/10.1007/BF02933060 |
Publisher: | Korean Society for Biotechnology and Bioengineering |
Keywords: | E. coli; Fermentation; HBcAg; pH; Rotational speed; Temperature |
Depositing User: | Nurul Ainie Mokhtar |
Date Deposited: | 19 Sep 2016 09:45 |
Last Modified: | 19 Sep 2016 09:45 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/BF02933060 |
URI: | http://psasir.upm.edu.my/id/eprint/34502 |
Statistic Details: | View Download Statistic |
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