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MALDI-target integrated platform for affinity-captured protein digestion


Ahmad Tajudin, Asilah and Adler, Belinda and Ekstrom, Simon and Marko-Varga, Gyorgy and Malm, Johan and Lilja, Hans and Laurell, Thomas (2014) MALDI-target integrated platform for affinity-captured protein digestion. Analytica Chimica Acta, 807. pp. 1-8. ISSN 0003-2670; ESSN: 1873-4324


To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1016/j.aca.2013.08.051
Publisher: Elsevier
Keywords: SPE; MALDI; Proteomics; PSA; Immunoaffinity; Digestion
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 15 Dec 2015 03:02
Last Modified: 15 Dec 2015 03:02
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.aca.2013.08.051
URI: http://psasir.upm.edu.my/id/eprint/34429
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