Citation
Ahmad Tajudin, Asilah and Adler, Belinda and Ekstrom, Simon and Marko-Varga, Gyorgy and Malm, Johan and Lilja, Hans and Laurell, Thomas
(2014)
MALDI-target integrated platform for affinity-captured protein digestion.
Analytica Chimica Acta, 807.
pp. 1-8.
ISSN 0003-2670; ESSN: 1873-4324
Abstract
To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences |
| DOI Number: | https://doi.org/10.1016/j.aca.2013.08.051 |
| Publisher: | Elsevier |
| Keywords: | SPE; MALDI; Proteomics; PSA; Immunoaffinity; Digestion |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 15 Dec 2015 03:02 |
| Last Modified: | 15 Dec 2015 03:02 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.aca.2013.08.051 |
| URI: | http://psasir.upm.edu.my/id/eprint/34429 |
| Statistic Details: | View Download Statistic |
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