Citation
Abstract
A process for producing lipase by microorganisms on the industrial scale, wherein Escherichia coli microorganism harbouring lipase, preferably thermostable lipase, is fermented under batch or fed batch conditions, in a Luria Bertani (LB) broth supplemented with citric acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, NH4Cl and glucose, using a trace metal solution containing hydrochloric acid, FeSO4.7H2O, ZnSO4.7H20, CaCl2.H20, MnSO4.5H20, CuSO4.5H2O, AlCl.6H2O, and H3BO3, and a feeding nutrient solution containing glucose, yeast extract, MgSO4.H2O, KH2PO4, Citric Acid, (NH4)2SO4, Na2HPO4.12H2O, MgCl2, and NH4Cl, at dissolved oxygen tension of 30-50 %, at temperature of 30-40 °C, while maintaining the pH between 6 and 8, with a glucose content of 15-25 g/L, with isopropylthiogalactoside.
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Additional Metadata
Item Type: | Patent |
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Application Number: | PI2013700818 |
Filing Date: | 2013-05-20 |
Divisions: | Faculty of Biotechnology and Biomolecular Sciences |
Keywords: | Producing lipase; Microorganisms |
Depositing User: | Mohd Hafiz Che Mahasan |
Date Deposited: | 17 Mar 2015 03:54 |
Last Modified: | 27 Sep 2016 01:19 |
URI: | http://psasir.upm.edu.my/id/eprint/33645 |
Statistic Details: | View Download Statistic |
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