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Isolation and development of DNA microsatellite markers for the river catfish (Mystus nemurus)


Citation

Hoh, Boon Peng and Siraj, Siti Shapor and Tan, Soon Guan and Yusoff, Khatijah (2007) Isolation and development of DNA microsatellite markers for the river catfish (Mystus nemurus). Asian Fisheries Science, 20 (1). pp. 41-53. ISSN 0116-6514

Abstract

The microsatellite markers for Mystus nemurus were isolated using the 5’ anchored PCR procedure. A degenerate primer was designed for the construction of the genomic library. Fourteen clones were sequenced and revealed a total of 45 microsatellite repeats. From these, 20 specific primer pairs were designed and employed to characterize unrelated fishes from six populations of M. nemurus. Thirteen loci were found to be polymorphic. The allelic frequencies ranged from 0.007 to 0.800, while the levels of heterozygosity ranged from 0.0563 (MnBp5-1-115b) to 0.8714 (MnBp5-1-12a).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Publisher: Asian Fisheries Society
Keywords: River catfish; Mystus nemurus; DNA microsatellite markers
Depositing User: Umikalthom Abdullah
Date Deposited: 01 Oct 2015 00:22
Last Modified: 01 Oct 2015 00:23
URI: http://psasir.upm.edu.my/id/eprint/32536
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