Citation
Hoh, Boon Peng and Siraj, Siti Shapor and Tan, Soon Guan and Yusoff, Khatijah
(2007)
Isolation and development of DNA microsatellite markers for the river catfish (Mystus nemurus).
Asian Fisheries Science, 20 (1).
pp. 41-53.
ISSN 0116-6514
Abstract
The microsatellite markers for Mystus nemurus were isolated using the 5’ anchored PCR procedure. A degenerate primer was designed for the construction of the genomic library. Fourteen clones were sequenced and revealed a total of 45 microsatellite repeats. From these, 20 specific primer pairs were designed and employed to characterize unrelated fishes from six populations of M. nemurus. Thirteen loci were found to be polymorphic. The allelic frequencies ranged from 0.007 to 0.800, while the levels of heterozygosity ranged from 0.0563 (MnBp5-1-115b) to 0.8714 (MnBp5-1-12a).
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Science |
| Publisher: | Asian Fisheries Society |
| Keywords: | River catfish; Mystus nemurus; DNA microsatellite markers |
| Depositing User: | Umikalthom Abdullah |
| Date Deposited: | 01 Oct 2015 00:22 |
| Last Modified: | 01 Oct 2015 00:23 |
| URI: | http://psasir.upm.edu.my/id/eprint/32536 |
| Statistic Details: | View Download Statistic |
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