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The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids


Citation

Abdul Samad, Norani and Ari, Zainab (1993) The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids. Pertanika Journal of Tropical Agricultural Science, 16 (2). pp. 157-160. ISSN 0126-6128

Abstract / Synopsis

Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions at 0.104 llg/ml. The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron microscopy test.


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Additional Metadata

Item Type: Article
Divisions: Universiti Pertanian Malaysia
Publisher: Universiti Putra Malaysia Press
Depositing User: Nasirah Abu Samah
Date Deposited: 24 Nov 2009 04:57
Last Modified: 01 Sep 2015 03:40
URI: http://psasir.upm.edu.my/id/eprint/3186
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