Citation
Abdul Samad, Norani and Ari, Zainab
(1993)
The Use of Antibody-sensitized Latex to detect Cymbidium Mosaic Virus in Orchids.
Pertanika Journal of Tropical Agricultural Science, 16 (2).
pp. 157-160.
ISSN 0126-6128
Abstract
Latex agglutination test (L) and a modification of the latex test, protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions at 0.104 llg/ml. The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron microscopy test.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Universiti Pertanian Malaysia |
| Publisher: | Universiti Putra Malaysia Press |
| Depositing User: | Nasirah Abu Samah |
| Date Deposited: | 24 Nov 2009 04:57 |
| Last Modified: | 01 Sep 2015 03:40 |
| URI: | http://psasir.upm.edu.my/id/eprint/3186 |
| Statistic Details: | View Download Statistic |
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