A Spectrophotometric Method for the Determination of Glucose with Glucose Oxidase [E.C. 1.11.1.7] Using Titanium (IV)-4-(2'-pyridylazo)Resorcinol Reagent

Citation

Ampon, Kamaruzaman and Wan Yunus, Wan Md Zin and Salleh, Abu Bakar (1994) A Spectrophotometric Method for the Determination of Glucose with Glucose Oxidase [E.C. 1.11.1.7] Using Titanium (IV)-4-(2'-pyridylazo)Resorcinol Reagent. Pertanika Journal of Science & Technology, 2 (1). pp. 47-53. ISSN 0128-7680

Abstract

A mixture of Ti(IV) and 4-(2-pyridylazo) resorcinol was found to be useful in the spectrophotometric determination of trace amounts of hydrogen peroxide. The absorbance of the complex formed at 508 nm was proportional to the concentration of hydrogen peroxide added. The reagent was applied to the assay of glucose through coupling with glucose oxidase which produces HP2' This method of glucose determination was rapid, convenient and showed minimal interference from reducible substances.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Environmental Studies
Publisher:	Universiti Putra Malaysia Press
Keywords:	spectrophotometric determination, hydrogen peroxide, Ti-PAR reagent, glucose oxidase
Depositing User:	Nur Izzati Mohd Zaki
Date Deposited:	24 Nov 2009 01:54
Last Modified:	27 May 2013 07:05
URI:	http://psasir.upm.edu.my/id/eprint/3107
Statistic Details:	View Download Statistic

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