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Efficiency of four Malaysian commercial disinfectants on removing Listeria monocytogenes biofilm


Citation

Fouladynezhad, Nina and Afsah Hejri, Leili and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2013) Efficiency of four Malaysian commercial disinfectants on removing Listeria monocytogenes biofilm. International Food Research Journal, 20 (3). pp. 1485-1490. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Listeria monocytogenes(L. monocytogenes) is a gram positive food-borne pathogen that is able to form biofilm on food factory surfaces. Formation of biofilm makes the bacteria much more resistance to environmental stresses such as disinfectant. The extracellular polymeric matrix (biofilm structure) which is mostly comprised of sticky extracellular polysaccharides (EPS) and proteins can protect bacteria in a harsh condition. The efficiency of four disinfectants on removing L. monocytogenes biofilm was investigated. Five concentration levels (100, 50,25, 12.5, and 6.25%) of disinfectants were tested. In the microtitre assay, the optical density at 595 nm CV-OD 595 value, was used to measure the amount of remained biofilm after 24 h. Results showed that disinfectants did not have significant effect on removing L. monocytogenes biofilm. Formation of L. monocytogenes biofilm significantly decreased the efficiency of disinfectants. Biofilm produced by strain number 9 showed higher resistance to disinfectant. Low concentrations (<50%) of disinfectants did not show significant effect on removing L. monocytogenes biofilm.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Biofilm; Listeria monocytogenes; Disinfectant
Depositing User: Khairil Ridzuan Khahirullah
Date Deposited: 04 Dec 2014 02:41
Last Modified: 02 Jun 2015 01:48
URI: http://psasir.upm.edu.my/id/eprint/30515
Statistic Details: View Download Statistic

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