Citation
Fouladynezhad, Nina and Afsah Hejri, Leili and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son
(2013)
Efficiency of four Malaysian commercial disinfectants on removing Listeria monocytogenes biofilm.
International Food Research Journal, 20 (3).
pp. 1485-1490.
ISSN 1985-4668; ESSN: 2231-7546
Abstract
Listeria monocytogenes(L. monocytogenes) is a gram positive food-borne pathogen that is
able to form biofilm on food factory surfaces. Formation of biofilm makes the bacteria much
more resistance to environmental stresses such as disinfectant. The extracellular polymeric
matrix (biofilm structure) which is mostly comprised of sticky extracellular polysaccharides
(EPS) and proteins can protect bacteria in a harsh condition. The efficiency of four disinfectants
on removing L. monocytogenes
biofilm was investigated. Five concentration levels (100, 50,25, 12.5, and 6.25%) of disinfectants were tested. In the microtitre assay, the optical density
at 595 nm CV-OD 595
value, was used to measure the amount of remained biofilm after 24 h.
Results showed that disinfectants did not have significant effect on removing L. monocytogenes biofilm. Formation of
L. monocytogenes biofilm significantly decreased the efficiency of disinfectants. Biofilm produced by strain number 9 showed higher resistance to disinfectant.
Low concentrations (<50%) of disinfectants did not show significant effect on removing L. monocytogenes biofilm.
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