Citation
Lye, Ying Ling and Afsah Hejri, Leili and Chang, Wei San and Loo, Yuet Ying and Soopna, Puspanadan and Kuan, Chee Hao and Mohd Shahril, Najwa and Rukayadi, Yaya and Khatib, Alfi and Tang, John Yew Huat and Nishibuchi, Mitsuaki and Nakaguchi, Yoshitsugu and Radu, Son
(2013)
Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk.
International Food Research Journal, 20 (2).
pp. 1001-1005.
ISSN 1985-4668; ESSN: 2231-7546
Abstract
E. coli
O157:H7 is associated with life threatening diseases such as hemorrhagic colitis (HC),
hemolytic uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). Raw
milk is considered a high risk food as it is highly nutritious and serves as an ideal medium for
bacterial growth. The aim of this study was to investigate the prevalence of
E. coli
O157:H7
in raw cow, goat and buffalo milk samples. MPN-PCR method targeting the major virulence
rfbE
gene and
fliCH
7
gene of
E. coli
O157:H7 was used. Total of 177 raw milk samples were
collected from local dairy farms in the state of Selangor, Malaysia. The highest prevalence
of
E. coli
O157:H7 was found in raw cow milk (8.75%) followed by raw goat milk (7.32%)
and raw buffalo milk (1.79%). The estimated quantity of
E. coli
O157:H7 in raw cow, goat
and buffalo milk ranged from <30 MPN/g to 120 MPN/g. In raw cow and goat milk samples
examined contain
E. coli
O157:H7 microbial load ranged from 30 to 120 MPN/g and 30 to 36
MPN/g, respectively.
E. coli
O157:H7 microbial load in buffalo milk samples was found to be
the lowest, only 30 MPN/g. Results of this research provide useful information on biosafety of
E. coli
O157:H7 in raw milk marketed in Malaysia.
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