Citation
Wong, B. Y. and Tan, Chin Ping and Ho, C. W.
(2013)
Effect of solid-to-solvent ratio on phenolic content and antioxidant capacities of “ Dukung Anak” (Phyllanthus niruri).
International Food Research Journal, 20 (1).
pp. 325-330.
ISSN 1985-4668; ESSN: 2231-7546
Abstract
The objective of this study was to evaluate the effects of solid-to-solvent ratio (1:5. 1:10, 1:15
and 1:20) on the extraction of phenolic compounds (TPC and TFC) and antioxidant capacity
(ABTS and DPPH radical scavenging capacity) of
P. niruri
. Solid-to-solvent ratio showed
a significant effect for both phenolic compounds (TPC and TFC) and antioxidant capacity
(ABTS and DPPH radical scavenging capacity) with 1:20 was the condition for extracting the
highest of phenolic compounds (TPC and TFC) with a value of 5788.7 mg GAE/100 g DW and
1906.5 mg CE/100 g DW, respectively and exhibited high antioxidant capacities (ABTS and
DPPH radical scavenging capacities) with a value of 0.820 mM and 1.598 mM, respectively
among the four levels studied. TPC was positively and significantly correlated with ABTS and
DPPH (r=0.999 and r=0.999) under the effects of solid-to-solvent ratio as compared to TFC,
positively and strongly correlated (r=0.865 and r=0.868) with ABTS and DPPH.
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