Citation
Abstract
Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity (P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Food Science and Technology Faculty of Agriculture Faculty of Science Faculty of Veterinary Medicine |
| DOI Number: | https://doi.org/10.4137/MBI.S10402 |
| Publisher: | Libertas Academica |
| Keywords: | Phytase; Recombinant; Optimization; Escherichia coli; Response surface methodology; Cultivation conditions |
| Depositing User: | Khairil Ridzuan Khahirullah |
| Date Deposited: | 14 Jul 2014 03:06 |
| Last Modified: | 23 Dec 2016 08:22 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.4137/MBI.S10402 |
| URI: | http://psasir.upm.edu.my/id/eprint/30427 |
| Statistic Details: | View Download Statistic |
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