First molecular characterization of Giardia duodenalis from goats in Malaysia

Citation

Lim, Yvonne A. L. and Mahdy, Mohammed A. K. and Tan, Tiong Kai and Goh, Xiang Ting and Jex, Aaron R. and Nolan, Matthew J. and Kumar Sharma, Reuben Sunil and Gasser, Robin B. (2013) First molecular characterization of Giardia duodenalis from goats in Malaysia. Molecular and Cellular Probes, 27 (1). pp. 28-31. ISSN 0890-8508; ESSN: 1096-1194

Abstract

In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (∼200 bp) from 21 of 310 (6.8%) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the ‘zoonotic’ assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Veterinary Medicine
DOI Number:	https://doi.org/10.1016/j.mcp.2012.08.006
Publisher:	Elsevier
Keywords:	Giardia duodenalis; Small subunit of the nuclear ribosomal RNA (SSU) gene; Goats; Malaysia.
Depositing User:	Azizan Arshad
Date Deposited:	09 Oct 2014 06:10
Last Modified:	01 Oct 2015 03:51
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.mcp.2012.08.006
URI:	http://psasir.upm.edu.my/id/eprint/29914
Statistic Details:	View Download Statistic

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