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High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia


Citation

Weng, Poh Leng and Ramli, Ramliza and Shamsudin, Mariana Nor and Cheah, Yoke Kqueen and Awang Hamat, Rukman (2013) High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multi locus sequence typing from a hospital in Malaysia. BioMed Research International, 2013. art. no. 938937. pp. 1-6. ISSN 2314-6133; ESSN: 2314-6141

Abstract

Little is known on the genetic relatedness and potential dissemination of particular enterococcal clones in Malaysia. We studied the antibiotic susceptibility profiles of Enterococcus faecium and Enterococcus faecalis and subjected them to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). E. faecium and E. faecalis displayed 27 and 30 pulsotypes, respectively, and 10 representative E. faecium and E. faecalis isolates (five each) yielded few different sequence types (STs): ST17 (2 isolates), ST78, ST203, and ST601 for E. faecium, and ST6, ST16, ST28, ST179, and ST399 for E. faecalis. Resistance to tazobactam-piperacillin and ampicillin amongst E. faecium isolates was highly observed as compared to E. faecalis isolates. All of the isolates were sensitive to vancomycin and teicoplanin. The presence of epidemic and nosocomial strains of selected E. faecium STs: 17, 78, and 203 and E. faecalis ST6 as well as high rates of resistance to multiple antibiotics amongst E. faecium isolates is of a particular concern.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1155/2013/938937
Publisher: Hindawi Publishing Corporation
Keywords: PFGE; Antibiotic resistance; Enterococci; VSE; MLST
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 08 Dec 2015 07:16
Last Modified: 08 Dec 2015 07:16
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2013/938937
URI: http://psasir.upm.edu.my/id/eprint/29560
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