Citation
Liew, Yun Khoon and Neela, Vasantha Kumari and Awang Hamat, Rukman and Amin Nordin, Syafinaz and Chong, Pei Pei
(2013)
Modified silver staining in 2DE improves protein detection even at extremely low sample concentration.
Electrophoresis, 34 (3).
pp. 397-400.
ISSN 0173-0835; ESSN: 1522-2683
Abstract
The typical concentration of protein loaded varies from 0.13 to 1.40 μg/μL for a classical silver staining method in 2DE gel. Here, we present a simple modified classical silver staining method by modifying the silver impregnation and development reaction steps. This modified method detects the protein spots at extremely low loaded concentrations, ranging from 0.0048 to 0.0480 μg/μL. We recommend this modified silver staining as an excellent method for the limited biological samples used for silver-stained 2DE analysis. Altogether, the protocol takes close to two days from first dimension separation to second dimension separation, followed by silver staining, scanning, and analysis.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Medicine and Health Science |
| DOI Number: | https://doi.org/10.1002/elps.201200380 |
| Publisher: | Wiley-VCH Verlag |
| Keywords: | 2DE; Protein spots; Silver stain |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 08 Dec 2015 04:05 |
| Last Modified: | 08 Dec 2015 04:06 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/elps.201200380 |
| URI: | http://psasir.upm.edu.my/id/eprint/29541 |
| Statistic Details: | View Download Statistic |
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