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Herbal sensitivity of Pseudomonas bacteria isolated from cultured tilapia with useful applications in vaccine preparation.


Citation

Sulaiman, Siti Fatimah and Mohd Salim, Khairul Afizi and Shamsudin, Mariana Nor and Abdelhadi, Yasser Mohamed (2013) Herbal sensitivity of Pseudomonas bacteria isolated from cultured tilapia with useful applications in vaccine preparation. Asian Journal of Animal and Veterinary Advances, 8 (2). pp. 383-388. ISSN 1683-9919

Abstract

The antibacterial activity of certain commercial antibiotics and common herbs was evaluated against pathogenic Pseudomonas fluorescens, Pseudomonas putida and Pseudomonas aeruginosa isolated from Malaysian and Egyptian cultured fish, mainly tilapia. A suspension of freshly cultured isolates was prepared (with 0.5 OD) and 100 μL of this suspension was spread over the Muller’s Hinton agar plates. The antibiotic discs were inoculated on each cultured plate while the herbal extracts were soaked on Whatman filter paper (20 μL each) that have been cut into discs and later inserted on to bacteria-cultured plates to screen their sensitivity to both antibiotics and herbs. Double-fold dilution was used to determine the Minimal Inhibitory Concentration (MIC) for the effective herbs at 100, 50, 25, 12.5 and 6.25%. Results revealed high resistance of the tested bacteria against most of the screened antibiotics except Ciprofloxacin. With regard to herbal sensitivity, only Origanum vulgare showed effectiveness and inhibition zone against all isolates. The MIC ranged from 15-40% for both Egyptian and Malaysian isolates. Thus, Origanum vulgare is recommended as a feed additive for cultured fish and can also be applied for inactivated and live-attenuated Pseudomonas vaccines’ preparation.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.3923/ajava.2013.383.388
Publisher: Academic Journals Inc
Keywords: Pseudomonas fluorescens; P. putida; P. aeruginosa; Herbs; Origanum vulgare.
Depositing User: Norhazura Hamzah
Date Deposited: 18 Jul 2014 08:11
Last Modified: 05 Feb 2016 01:01
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ajava.2013.383.388
URI: http://psasir.upm.edu.my/id/eprint/29510
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