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Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers


Citation

Ravanfar, Seyed Ali and Abdul Aziz, Maheran and Shabanimofrad, Mahmoodreza and Samarfard, Samira (2013) Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers. Electronic Journal of Biotechnology, 16 (5). pp. 1-10. ISSN 0717-3458

Abstract

Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture were transferred into disposable plastic pots containing sterilized potting mixture consisting of (peatgro™) + coconut dust (2:1) and maintained in a growth chamber. Results: After one month, the hardened plantlets were transferred and maintained in a transgenic greenhouse. After four months of acclimatization in the transgenic greenhouse, the efficacy of HSP101 gene in increasing the heat tolerance of the transgenic broccoli was evaluated. Results showed that the transgenic plants could survive and performed normally, producing flower heads even at the highest tested temperature of 34°C. Seven transgenic broccoli lines with different gene copy number of the AtHSP101 gene as well as the control plant were assessed for genetic diversity using inter simple sequence repeat (ISSR) markers. Conclusions: ISSR results showed polymorphism and phylogenetic relationship between the transgenic and non-transgenic (control) Brassica oleracea cv. Green Marvel.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Agriculture
DOI Number: https://doi.org/10.2225/vol16-issue5-fulltext-10
Publisher: Pontificia Universidad Catolica de Valparaiso
Keywords: AtHSP101 gene; Brassica oleracea; ISSR; Transgenic broccoli
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 25 Apr 2016 04:19
Last Modified: 25 Apr 2016 04:19
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2225/vol16-issue5-fulltext-10
URI: http://psasir.upm.edu.my/id/eprint/29294
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