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Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis.


Citation

James Antony, Jessica Jeyanthi and Chan, Lai Keng and Mahmood, Maziah and Subramaniam, Sreeramanan (2013) Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis. Applied Biochemistry and Biotechnology, 171 (2). pp. 315-329. ISSN 0273-2289; ESSN: 1559-0291

Abstract

Regrowth of the cryopreserved protocorm-like bodies (PLBs) of Dendrobium Bobby Messina was assessed based on the plant vitrification solution 2 (PVS2) optimisation conditions. The optimized protocol obtained based on TTC spectrophotometrical analysis and growth recovery were 3–4 mm of PLBs size precultured in 0.2 M sucrose for 1 day, treated with a mixture of 2 M glycerol and 0.4 M sucrose supplemented with half-strength liquid MS media at 25 °C for 20 min and subsequently dehydrated with PVS2 at 0 °C for 20 min prior to storage in liquid nitrogen. Following rapid warming in a water bath at 40 °C for 90 s, PLBs were treated with unloading solution containing half-strength liquid MS media supplemented with 1.2 M sucrose. Subsequently, the PLBs were cultured on half-strength semi-solid MS media supplemented with 2 % (w/v) sucrose without any growth regulators and resulted in 40 % growth recovery. In addition, ascorbic acid treatment was used to evaluate the regeneration process of cryopreserved PLBs. However, growth recovery rates of non-cryopreserved and cryopreserved PLBs were 30 and 10 % when 0.6 mM ascorbic acid was added. Scanning electron microscopy analysis indicates that there are not much damages observed on both cryopreserved and non-cryopreserved PLBs in comparison to PLBs stock culture.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Humana Press
Keywords: Cryopreservation; Dendrobium Bobby Messina; Regeneration rates; Ascorbic acid.
Depositing User: Umikalthom Abdullah
Date Deposited: 29 May 2014 02:51
Last Modified: 05 Oct 2015 06:19
URI: http://psasir.upm.edu.my/id/eprint/28137
Statistic Details: View Download Statistic

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