Effect of temperature and pH on growth pattern of Ganoderma boninense from oil palm in Peninsular Malaysia

Mycelial growth of boninense from floating inoculum disks incubates in flasks swirled daily was significantly more abundant than that from submerged inoculum disks in static flasks. The optimum temperatures for mycelial grow in both solid and liquid media were between 27-30°C. The mycelial growth pattern in liquid medium showed an initial lag phase of little growth (1-3 days), then a phase of rapid growth (6-18 days) and finally a phase of autolysis and decline in dry weight. The pH of medium change correspondingly with the change in growth pattern. As mycelial growth increased, the pH of medium decreased and when mycelial growth decreased during prolonged incubation, pH of medium increased. Optimum pH for mycelial growth was between 3.7-5.0.

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