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Bioconversion of restaurant waste into polyhydroxybutyrate (PHB) by recombinant E. coli through anaerobic digestion


Citation

Eshtaya, Majd Khalid and Abdul Rahman, Nor'aini and Hassan, Mohd Ali (2013) Bioconversion of restaurant waste into polyhydroxybutyrate (PHB) by recombinant E. coli through anaerobic digestion. International Journal of Environment and Waste Management, 11 (1). pp. 27-37. ISSN 1478-9876; ESSN: 1478-9868

Abstract

The effect of temperature (30°C, 37°C, uncontrolled) and initial pH adjustment at pH 7 in the anaerobic digestion process was investigated to enhance the production of organic acids from restaurant waste. The highest organic acid level obtained was 39.6 g/L on the fifth day of fermentation conducted at 30°C and initial pH 7. The acids produced corresponded to 39.4% of the yield based on the initial concentration of substrate. The main organic acids produced were lactic and acetic acids. Using organic acids from fermented restaurant waste, recombinant Escherichia coli pnDTM2 gave PHB concentration, PHB content and PHB productivity of 9.2 g/L, 44% w/w and 0.54 g/L/h, respectively, in a pH stat fed-batch culture.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1504/IJEWM.2013.050521
Publisher: Inderscience Enterprises
Keywords: Anaerobic digestion; Organic acids; Polyhydroxybutyrate; Restaurant waste
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 20 Jun 2016 05:55
Last Modified: 20 Jun 2016 05:55
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1504/IJEWM.2013.050521
URI: http://psasir.upm.edu.my/id/eprint/28085
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