Citation
Abstract
This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol) 5, 15, 25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (M n) of P(3HB-co-3HV) copolymer reached the highest at 838 × 10 3 Da with polydispersity index (PDI) value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4). Tensile strength and Young's modulus of the copolymer containing 6-8 mol% 3HV were in the ranges of 13-14.4 MPa and 0.26-0.34 GPa, respectively, comparable to those of polyethylene (PE). Thus, Comamonas sp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials.
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Official URL or Download Paper: http://www.hindawi.com/journals/bmri/2013/237806/a...
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Engineering |
DOI Number: | https://doi.org/10.1155/2013/237806 |
Publisher: | Hindawi Publishing Corporation |
Keywords: | Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Comamonas sp. EB172; Volatile fatty acids; Carbon to nitrogen ratio |
Depositing User: | Nurul Ainie Mokhtar |
Date Deposited: | 20 Jun 2016 03:06 |
Last Modified: | 20 Jun 2016 03:06 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2013/237806 |
URI: | http://psasir.upm.edu.my/id/eprint/28068 |
Statistic Details: | View Download Statistic |
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