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An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli


Citation

Tan, Joo Shun and Ling, Tau Chuan and Mustafa, Shuhaimi and Tam, Yew Joon and Ramanan, Ramakrishnan Nagasundara and Ariff, Arbakariya (2013) An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli. Process Biochemistry, 48 (4). pp. 551-558. ISSN 1359-5113; ESSN: 1873-3298

Abstract

A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.79 g/L) secreted by E. coli in the integrated STB/EBA system was higher than that in a bioreactor with dispersed resin or a conventional batch bioreactor, cell growth (14.97 g/L) and α-PrIFN-2b production (867.4 μg/L) were significantly improved owing to the high efficiency of acetate removal from the culture. The production of α-PrIFN-2b in the integrated STB/EBA system was improved by 3-fold and 1.4-fold over that obtained in a conventional batch bioreactor and a bioreactor containing dispersed resins, respectively.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.procbio.2013.02.024
Publisher: Elsevier
Keywords: Bioreactors; Expanded bed adsorption; Fermentation; Acetic acid; α-Interferon-2b
Depositing User: Nabilah Mustapa
Date Deposited: 21 Apr 2016 06:08
Last Modified: 21 Apr 2016 06:08
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.procbio.2013.02.024
URI: http://psasir.upm.edu.my/id/eprint/28066
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