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Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM


Basri, Mahiran and Kassim, Mohd Azlan and Mohamad, Rosfarizan and Ariff, Arbakariya (2013) Optimization and kinetic study on the synthesis of palm oil ester using Lipozyme TL IM. Journal of Molecular Catalysis B: Enzymatic, 85-86. pp. 214-219. ISSN 1381-1177; ESSN: 1873-3158


Enzymatic synthesis of palm oil esters (POE) was carried out via alcoholysis of palm oil (PO) and oleyl alcohol (OA) catalyzed by Lipozyme TL IM. The optimum reaction conditions were: temperature: 60 °C; enzyme load: 24.7 wt%; substrate ratio: 1:3 (PO/OA), impeller speed: 275 rpm and reaction time: 3 h. At the optimum condition, the conversion of POE was 79.54%. Reusability study showed that Lipozyme TL IM could be used for 5 cycles with conversion above 50%. The alcoholysis reaction kinetic follows the Ping-Pong Bi-Bi mechanism characterized by the Vmax, Km(PO), and Km(OA) values of 32.7 mmol/min, 0.3147 mmol/ml and 0.9483 mmol/ml, respectively. The relationship between initial reaction rate and temperature was also established based on the Arrhenius law.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.molcatb.2012.09.013
Publisher: Elsevier
Keywords: Lipozyme TL IM; Enzymatic esterification; Kinetic study; Ping-Pong Bi-Bi model; Palm oil esters synthesis
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 07 Mar 2016 06:46
Last Modified: 07 Mar 2016 06:46
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.molcatb.2012.09.013
URI: http://psasir.upm.edu.my/id/eprint/28020
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