Citation
Abstract
Enzymatic synthesis of palm oil esters (POE) was carried out via alcoholysis of palm oil (PO) and oleyl alcohol (OA) catalyzed by Lipozyme TL IM. The optimum reaction conditions were: temperature: 60 °C; enzyme load: 24.7 wt%; substrate ratio: 1:3 (PO/OA), impeller speed: 275 rpm and reaction time: 3 h. At the optimum condition, the conversion of POE was 79.54%. Reusability study showed that Lipozyme TL IM could be used for 5 cycles with conversion above 50%. The alcoholysis reaction kinetic follows the Ping-Pong Bi-Bi mechanism characterized by the Vmax, Km(PO), and Km(OA) values of 32.7 mmol/min, 0.3147 mmol/ml and 0.9483 mmol/ml, respectively. The relationship between initial reaction rate and temperature was also established based on the Arrhenius law.
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| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Science Institute of Bioscience |
| DOI Number: | https://doi.org/10.1016/j.molcatb.2012.09.013 |
| Publisher: | Elsevier |
| Keywords: | Lipozyme TL IM; Enzymatic esterification; Kinetic study; Ping-Pong Bi-Bi model; Palm oil esters synthesis |
| Depositing User: | Nurul Ainie Mokhtar |
| Date Deposited: | 07 Mar 2016 06:46 |
| Last Modified: | 07 Mar 2016 06:46 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.molcatb.2012.09.013 |
| URI: | http://psasir.upm.edu.my/id/eprint/28020 |
| Statistic Details: | View Download Statistic |
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