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Effect of hypoxia on the response of canine mammary gland tumor cells to bovine lactoferrin, doxorubucin and recombinant human erythropoietin


Citation

Tan, Felina Peck Yen and How, Chee Wun and Teo, Guan Young and Abdullah, Rasedee (2010) Effect of hypoxia on the response of canine mammary gland tumor cells to bovine lactoferrin, doxorubucin and recombinant human erythropoietin. In: 5th Proceedings of the Veterinary Sciences, 5-8 Jan. 2010, Serdang, Selangor. (p. 74).

Abstract

The exact role of hypoxia in tumor biology remains controversial because there is no conclusive evident on its effect on tumors. There is concern that tumor hypoxia is one of the causes of chemoresistance in cancer cells. Recently, erythropoietin receptors have been found in human breast cancer cells indicating that recombinant human erythropoietin (rHuEPO) treatment of cancer-related anemia can influence the functions of the cells. Bovine lactoferrin (bLF) was also shown to have antiproliferative effects on cancer cells. The aim of this study was to determine the in vitro effects of rHuEPO, bLF and Doxorubicin (DOX) on a canine mammary gland tumor cell line, CMT-stylo cells, under hypoxic condition. The cells were treated with bLF, rHuEPO, DOX rHuEPO and DOX and bLF, rHuEPO combinations. These treated cells were subjected to MTT assay. The results showed that hypoxia lowered the proliferation rate of the CMT-stylo cells while combination treatments showed improved killing. Flow cytometry analysis showed that DOX had cytotoxic while bLF had antiproliferative effects on the CMT-stylo cells.


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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Veterinary Medicine
Keywords: Hypoxia; Canine mammary gland tumor; MTT assay; Flow cytometry; Doxorubicin; Bovine lactoferrin; Recombinant human erythropoietin.
Depositing User: Azizan Arshad
Date Deposited: 05 May 2014 02:06
Last Modified: 05 May 2014 02:16
URI: http://psasir.upm.edu.my/id/eprint/27660
Statistic Details: View Download Statistic

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