In vitro controlled release of an anticancer drug, chlorogenic acid using magnesium/aluminium layered double hydroxide as nanomatrix.

An anticancer agent, chlorogenìc acrd was intercaìated into 2D nanolamellae of Mg/Al-NO3-layered double hydroxide through the dìrect, co-p recipitation and indirect, ion exchangetechniques, for the formation of new nanocornposites, CMAC and CMAE, respectively. The loaading amount of chlorogenic acid in the nanocomposìtes was estìmated to be 37.3% for CMAE and 54.2% for CMAC and both nanocomposites show mesoporous- property. The basal spacing of CMAE and CMAC were expanded 1o 11,70 and 12.18 A, respectively compared to the host, suggesting that chiorogenates, the anions of chlorogenic acid were intercalated into the nanolamellae of Mg/Al-layered double hydroxide and arranged themselves in a horizontal monoìayer fashion with g0' angle from x ax¡s in CMAE and 50" angle for CMAC. The ìntercalation process was also conftrmed by x-ray diffraction and Fourier transform infrared studies and the intercalated chlorogenic acid was found to be thermally more stable than its free chlorogenic acid counterparts. The release of chlorogenate from the nanocomposites, CMAE and CMAC at pH 7.4 and 4.8 of phosphate buffered solutlon occurred in a controlled manner, governed by pseudo-second order release mechanism. ln vitro anti-cancer properties of both the synthesìzed nanocomposites CMAE and CMAC against various human cancer cells namely MCF-7, HeLa, llepG2, 4549 and normal fibroblast cells, 3T3 were investigated by cell cytotoxrcity assay. It was fourrd that the nanocomposites CMAE and CMAC, showed better cytotoxicity propeÉies aga¡nst the cancer cells particularly the liver cancer cells, HepG2 in a dose dependent manner and these nanocomposites did not produce any toxicity behavior in normal fibroblast cells. This preliminary investìgation suggest that the nanocomposites showed antitumor properties against cancer cells without showing toxicity to normal fibroblast cells, hence further pre-clinical studìes are needed to explore more on the potential of tire nanocomposite as an anticancer drug delivery system.

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