Mineral phosphate solubilization of rhizobia and endophytic nodule bacteria of legumes.

Nitrogen and phosphorous are two of the main elements required for plant growth. Beneficial microorganisms can supply nitrogen through biological nitrogen fixation and increase P uptake by P solubilization of mineral phosphates through the production of organic acids. Rhizobium is a well established biological nitrogen fixer but other potential characteristics that promote overall plant growth have been largely ignored. A study was carried on rhizobia and root nodule endophytes from legumes to determine their phosphate solubilizing ability using solid and broth media containing mineral phosphates. A total of 160 rhizobia and root nodule endophytes were tested. Only 42 isolates (26.25%) showed phosphate solubilization activity using solid agar media and from these, a further 14 isolates were studied based on growth in various liquid mineral phosphate media. Isolate GM 119, a root nodule endophyte from Glycine max, showed the best result for phosphate solubilization on media containing Ca,(P04)2.

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