Towards the development of a protocol for cryopreservation of petai (parkia speciosa hassk.) zygotic embryos.

Citation

Lim, S.P. and R.S., Uma (2001) Towards the development of a protocol for cryopreservation of petai (parkia speciosa hassk.) zygotic embryos. In: Malaysian Society of Plant Physiology Conference, 29 Sept. 2001, Seri Kembangan, Malaysia. (pp. 175-178). (Unpublished)

Abstract

This study was carried out to evaluate the potential of cryopreservation technique for germplasm conservation of petai zygotic embryos. The effect of various desiccation periods and different concentration of sucrose pretreatment on survival of petai embryos in liquid nitrogen (LN) was evaluated. The result shows that non-pretreated petal embryos were sensitive to desiccation whereby a reduction of moisture content to below 16% decreased survival percentage to less than 48%. On freezing, no embryos survived irrespective of their moisture content. Sucrose pretreatment was found to enhance desiccation and freezing tolerance in petai embryos whereby survival percentage of approximotely 90"/6 and 50% was observed, without and with LN exposure respectively.

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Additional Metadata

Item Type:	Conference or Workshop Item (Paper)
Divisions:	Faculty of Agriculture
Notes:	Fulltext are available at Special Collection Division Office.
Keywords:	Cryopreservation; Parkia speciosa hassk.; Petai; Zygotic embryos.
Depositing User:	Erni Suraya Abdul Aziz
Date Deposited:	22 Jan 2014 00:23
Last Modified:	13 Jun 2014 08:24
URI:	http://psasir.upm.edu.my/id/eprint/26811
Statistic Details:	View Download Statistic

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