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Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia.


Citation

Bamaiyi , Pwaveno Huladeino and Hassan, Latiffah and Bejo, Siti Khairani and Mohamed, Zainal Abidin and Mahmod, Ramlan and Krishnan, N. and Adzhar, A. and Abdullah, N. and N.H.M, Hamidah and M.M, Norsuhanna and Hashim, S.N. (2011) Isolation and polymerase chain reaction confirmation of Brucella melitensis from goats in Malaysia. In: 2nd International Symposium on Zoonoses and Emerging Infectious Diseases , 2011, Kuala Lumpur, Malaysia. (p. 30).

Abstract

A study was carried out to isolate Brucella melitensis using established bacteriological techniques and the Polymerase Chain Reaction in previously known positive goat farms in Selangor, Negeri Sembilan, Melaka and Pulau Pinang states of Malaysia. A total of 7 isolates were recovered from vaginal swabs, spleen and uterine fluid using standard biochemical tests with vaginal swabs yielding the highest isolates of 4 (57.14%) followed by the spleen 2 (28.57%) and uterine fluid 1(14.29%). No isolates were recovered from the lymph nodes. PCR confirmed all 7 isolates as B. melitensis


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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Veterinary Medicine
Keywords: Polymerase Chain Reaction; Brucella; Goats.
Depositing User: Azizan Arshad
Date Deposited: 11 Oct 2013 08:04
Last Modified: 11 Oct 2013 08:04
URI: http://psasir.upm.edu.my/id/eprint/25707
Statistic Details: View Download Statistic

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