Citation
Tengku Adnan, Tengku Adnin and Augustin, M. A. and Mohd Ghazali, Hasanah
(1986)
Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I.
Pertanika, 9 (2).
pp. 219-224.
Abstract
Polyphenoloxidase (PPO) was z50lated from starfruit (Averrhoa carambola, L.) and purified
by acetone precipitation and dialysis. The optimum pH for the aCl}tone purified PPO catalysed
oxidation of catechol was found to be pH 7.2 Heat 'inactivation studies showed that the enzyme was
heat-labile. Haif-Hvesfor PPO 'inactiivation at 70°C and 75°C were found to be 4.6 min and 3.1 min
respectively. The Michaeline constant (K j and V for catechol with PPO was O. 061M and 20 U ml-1
respectively.
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