Citation
Abstract
Polysaccharides were isolated from the fibrous pulp of bambangan (Mangifera pajang Kort.). Neutral and acidic polysaccharides were separated using DEAE-Cellulose. Size exclusion chromatography analyses showed that the average molecular weight (MW) of the neutral M. pajang polysaccharides (F1) was approximately 7 kDa, and those of three acidic polysaccharides (F2, F3 and F4) were approximately 13, 24 and 9 kDa, respectively. The monosaccharide compositions of these polysaccharides were determined using high performance liquid chromatography. F1 contained erythrose, rhamnose, arabinose, mannose, fructose and glucose (5, 7, 21, 42, 4 and 21 mg/100 mg fraction respectively), F2 consisted of rhamnose, xylose and arabinose (33, 7 and 51 mg/100 mg fraction respectively), F3 consisted of fructose (14 mg/100 mg fraction) and glucose (72 mg/100 mg fraction), and F4 comprised arabinose, mannose, fructose and glucose (32, 36, 2 and 10 mg/100 mg fraction respectively). Results of Fourier transform infrared spectroscopy and the monosaccharide compositions suggested that the fibrous pulp of M. pajang fruit consisted of heteropolysaccharide and belonged to α and β–type of the pyran group. Additionally, crude polysaccharide and its fractions showed strong antioxidant activities. The acidic polysaccharides had the highest antioxidant activity and should be considered as a prospective antioxidant.
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Medicine and Health Science Faculty of Biotechnology and Biomolecular Sciences Faculty of Food Science and Technology Halal Products Research Institute |
DOI Number: | https://doi.org/10.1016/j.lwt.2012.04.002 |
Publisher: | Elsevier |
Keywords: | Mangifera pajang Kort.; Polysaccharides; Purification; Characterization; Antioxidant activity. |
Depositing User: | Nur Farahin Ramli |
Date Deposited: | 24 Mar 2014 06:59 |
Last Modified: | 22 Sep 2015 00:57 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.lwt.2012.04.002 |
URI: | http://psasir.upm.edu.my/id/eprint/24516 |
Statistic Details: | View Download Statistic |
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