Citation
Abstract
Nigella sativa has been used for centuries in Asia, Middle East and Africa to promote health and fight diseases. In this study, the anti-cancer effects of thymoquinone rich fraction (TQRF) extracted from N. sativa seeds using supercritical fluid extraction (SFE) system and commercially available thymoquinone (TQ) on colon cancer (HT29), lymphoblastic leukemia (CEMSS) and promyelocytic leukemia (HL60) cells lines were investigated. The concentration that gave 50% inhibition of cell viability (IC50) of HT29, CEMSS and HL60 cells treated with TQRF were 400, 350 and 250 µg/ml, respectively. Meanwhile, the IC50 of TQ was 8, 5 and 3 µg/ml, respectively. Cell cycle analysis shows the increment of apoptosis in a time-dependent manner. However, both TQRF and TQ were not able to arrest the cell cycle phases of the cells. Apoptosis was the main mode of HT29, CEMSS and HL60 cells death induced by both TQRF and TQ. Our findings support the potential use of TQRF and TQ for the treatment of colon cancer and leukemia.
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Official URL or Download Paper: http://www.academicjournals.org/journal/JMPR
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Medicine and Health Science Institute of Bioscience |
| Publisher: | Academic Journals |
| Keywords: | Nigella sativa; Thymoquinone; Supercritical fluid extraction system; Colon cancer; Lymphoblastic leukemia; Promyelocytic. leukemia, cell cycle. |
| Depositing User: | Nur Farahin Ramli |
| Date Deposited: | 30 Mar 2014 03:30 |
| Last Modified: | 22 Sep 2015 07:44 |
| URI: | http://psasir.upm.edu.my/id/eprint/24493 |
| Statistic Details: | View Download Statistic |
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