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Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression


Citation

Yeap, Swee Keong and Omar, Abdul Rahman and Ho, Wan Yong and Beh, Boon Kee and Ali, Abdul Manaf and Mohammed Alitheen, Noorjahan Banu (2011) Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression. African Journal of Biotechnology, 10 (52). art. no. E25A36A35246. pp. 10744-10751. ISSN 1684–5315

Abstract

Rhaphidophora korthalsii methanol extract was previously found as a potential in vitro immunomodulating agent. In this study, the in vitro immunomodulatory activity of R. korthalsii methanol extract on mice splenocyte were quantified through evaluating T/NK cell population, cytokine expression (IL-2 and IFN-) and cytotoxicity against Yac-1 cell. In vitro MTT proliferation study showed that R. korthalsii methanol extract stimulate significant proliferation of splenocyte, thymocyte and bone marrow cell at 25 g/ml after 72 h incubation as compared to rIL-2. Besides, 25 g/ml of extract also enhanced T-helper 1 cytokine (IL-2 and IFN-) secretion, natural killer cell (NK1.1+CD3-) population and cytotoxicity against natural killer sensitive Yac-1 leukemic cell line. It was concluded that R. korthalsii methanol extract is a potential immunomodulator agent that could activate the natural killer cell.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Academic Journals
Keywords: Cytotoxicity; Immunomodulation; In vitro; Rhaphidophora korthalsii
Depositing User: Nur Farahin Ramli
Date Deposited: 25 Feb 2014 00:05
Last Modified: 27 Apr 2016 03:14
URI: http://psasir.upm.edu.my/id/eprint/24113
Statistic Details: View Download Statistic

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